FDA-licensed LAL Reagents
Limulus amebocyte lysate (LAL) is an aqueous extract of blood cells (amebocytes) from the Atlantic horseshoe crab, Limulus polyphemus. Comprised of proteins, LAL is used to detect the presence of endotoxins, a cell wall component of Gram-negative bacteria that causes a pyrogenic response (fever) and symptoms of septic shock. Even if dead, these components trigger a response in LAL, causing it to clot and coagulate.
Endosafe® Trillium™ Recombinant Cascade Reagent (rCR) – Vials Product Specifications:
- Quantitative range of 0.001 to 100 EU/mL, offering exceptional sensitivity and reliability
- Eliminates the potential for 1,3-β-D-glucans, reducing the risk of false positives
- Reagent Stability up to 4 hours at 2-8°C
- Pack of 6, 32-test vials
Kinetic Turbimetric LAL Reagent
Charles River Endosafe two kinetic turbidimetric LAL endotoxin test reagents, KTA and KTA2, yield quantitative endotoxin values when used with a microplate reader equipped with endotoxin-measuring software. Both reagents are buffered to provide significant interference-resistance properties and are FDA-licensed for use in product release testing. Our kinetic turbidimetric endotoxin test reagents are buffered to give the best interference-resistance properties.
Charles River Endosafe traditional kinetic turbidimetric assay reagent (KTA) is suitable for both kinetic and gel-clot analyses and permits a direct correlation between LAL methods.
Charles River Endosafe second-generation reagent KTA2 offers one of the fastest turbidimetric LAL reaction times on the market, and our optimized formulations offer an unrivaled combination of sensitivity, linearity, and interference resistance properties with minimal assay times. Its superior turbidimetric formulation offers the performance of a kinetic chromogenic LAL test at the price of a turbidimetric reagent.
| Product | Sensitivity | Product Code |
|---|---|---|
| KTA 50-Test Vial (5.2 mL) |
0.015 EU/mL | R15015 |
| 0.03 EU/mL | R15003 | |
| 0.06 EU/mL | R15006 | |
| KTA2 50-Test Vial (5.2 mL) |
0.005 EU/ml | R19000 |
Gel-Clot Endotoxin Test Reagents
The gel-clot assay determines the presence of endotoxins through its clotting response, and is a quick and effective way to detect harmful endotoxins for your injectable pharmaceuticals. Endosafe® Rapid LAL Single-Test Vials contain FDA-licensed LAL to provide a simple, self-contained option for routine endotoxin detection in research samples, renal dialysis process water, and dialysate solutions.
Accurate gel-clot assay results ensure the safety of your pharmaceuticals. The gel-clot endotoxin test is a simple qualitative method best used for low-volume laboratories or for products such as those that are highly viscous or extremely colorful. Our gel-clot endotoxin test lysate features a firm gel over a wide range of sensitivities as the buffered reagent provides better interference resistance and higher accuracy for routine LAL testing. Find out if gel-clot is the best method for your lab.
| Product | Sensitivity EU/mL | Code |
|---|---|---|
| 50-test vial (5.2 mL) | 0.015 | R15015 |
| 0.03 | R15003 | |
| 0.06 | R15006 | |
| 0.125 | R11012 | |
| 0.25 | R11025 | |
| 10-test vial (1.2 mL) | 0.03 | R12003 |
| 0.06 | R12006 | |
| 0.125 | R12012 | |
| 0.25 | R12025 |
Traditional LAL Kinetic Chromogenic Assay
Endochrome-K™, our traditional LAL kinetic chromogenic assay (KCA) facilitates your endotoxin screening with its ease of use and unique reagent stability. Featuring an optimized 0.005 EU/mL sensitivity*, our traditional kinetic chromogenic assay reagent provides heightened sensitivity for more accurate results, greater linearity for endotoxin analyses, and superior interference resistance. Our traditional LAL kinetic chromogenic reagent is designed to enhance your kinetic chromogenic assay and rapidly deliver accurate results.
| Product | Code |
|---|---|
| Endochrome-K™ kit - 256 tests (sensitivity= 50-0.005 EU/ml) 8 X3.2 mL vials 2 X 10 ng control standard endotoxin 3 X 30 mL LAL reagent water |
R1708K |
| Endochrome-K™ (sensitivity= 50-0.005 EU/ml) 3.2 mL vials x100 |
R1700 |
| Endochrome-K™ (sensitivity= 50-0.005 EU/ml) 3.2 mL vials x10 |
R1710K |
| Endpoint chromogenic kit (140 tests) 5 X 1.4 mL vials of chromogenic LAL 1 X 10 mg vial of chromogenic substrate S-2423 2 X 2 ng vials of endotoxin 2 X 30 mL vials of LAL reagent water 1 X 15 mL 0.05 M vial of Tris buffer |
R160K |
LAL Assay Accessories
Charles River provides all the necessary endotoxin-free test tubes and accessory products required to run an LAL assay. All accessory products are provided with a certificate of quality certifying appropriate endotoxin levels. These high-quality accessories impart control and consistency to LAL assays and the LAL laboratory, thereby minimizing invalid results and repeat testing. A critical part of running a LAL assay is the choice of endotoxin-free test tubes and accessories that enable data collection free of artifacts and sources of interference. The selection of non-interfering LAL assay accessories for a bacterial endotoxin test is not only a pharmacopeial directive, but also a regulatory expectation. Accessories play a major role in the quality of results generated in the BET lab.
| Product | Packaging | Code |
|---|---|---|
| CSE – 500 ng per vial* | 6/pack | E110 |
| CSE – 10 ng per vial* | 6/pack | E120 |
| Positive control (for single test) | 25/pack | PC100 |
| Reference standard endotoxin (RSE) | E150 | |
| Extended CSE (CXE) Dilution Kit* 1 × 10 ng CSE 1 × 60 mL stabilizing solution 1 pack of 11 of 16 × 100 mm capped tubes |
E140 |
| Product | Packaging | Code |
|---|---|---|
| 30 mL bottle (< 0.001 EU/mL) | 12/case | W130 |
| 50 mL bottle (< 0.001 EU/mL) | 12/case | W120 |
| 100 mL bottle (< 0.001 EU/mL) | 12/case | W110 |
| 500 mL bottle (< 0.001 EU/mL) | 6/pack | W150 |
| Product | Packaging | Code |
|---|---|---|
| 5 mL 0.25 M Tris buffer | 6/pack | BT101 |
| 30 mL 0.1 M Tris buffer | 12/case | BT103 |
| 5.5 mL 0.1 M Tris buffer | 6/pack | BT105 |
| 30 mL 0.05 M Tris buffer | 12/case | BT106 |
| 4 mL 0.5 M MgSO4, 1 M Tris buffer | 6/pack | BC1000 |
| 30 mL bio-dispersing agent | 12/case | BD100 |
| Endotoxin-Specific (ES) Buffer | 6/pack | BG200 |
| Product | Code |
|---|---|
| 10,000 EU | EVV10K |
| 100,000 EU | EVV100K |
| 1 Million EU | EVV1M |
| 2.5 Million EU | EVV2.5M |
| 10 Million EU | EVV10M |
| Product | Packaging | Code |
|---|---|---|
| 10 × 75 mm capped flint glass tubes, boxed | 50/pack | T100 |
| 10 × 75 mm flint glass tubes, in foil | 50/pack | T200 |
| 10 × 75 mm borosilicate glass tubes, in foil (appropriate for use with tube readers) |
50/pack | T400 |
| 8 × 75 mm borosilicate glass tubes, in foil (only for use with tube readers) |
50/pack | T500 |
| 10 × 75 mm screw-cap borosilicate glass tubes, boxed | 70/pack | TL1200 |
| Product | Packaging | Code |
|---|---|---|
| 12 × 75 mm borosilicate glass tubes, in foil | 50/pack | TL1000 |
| 13 × 100 mm borosilicate glass tubes, in foil | 50/pack | T300 |
| 16 × 90 mm screw-cap borosilicate glass tubes, boxed | 70/pack | TL700 |
| 18 × 150 mm borosilicate glass tubes, in foil | 14/pack | T600 |
| Product | Packaging | Code |
|---|---|---|
| 1 mL, in foil | 5 packs | P100 |
| 2 mL, in foil | 5 packs | P200 |
| 5 mL, in foil | 5 packs | P500 |
| 10 mL, in foil | 5 packs | P1000 |
| Product | Packaging | Code |
|---|---|---|
| Eppendorf® pipette tips (2-200 μL,* individually wrapped) | 50/pack | D100EA |
| Eppendorf® pipette tips (2-200 μL,* individually wrapped) | 100/pack | D200 |
| Product | Code |
|---|---|
| 96-well polystyrene plate (certified to 0.005 EU/mL) | M9005 |
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